Molecular cell biology / Harvey Lodish, Arnold Berk, Chris A. Kaiser, Monty Krieger, Anthony Bretscher, Hidde Ploegh, Angelika Amon, Matthew P. Scott.

Includes bibliographical references and index.

Part I. Chemical and molecular foundations. Molecules, cells, and evolution. The molecules of life -- Genomes, cell architecture, and cell function -- Cells into tissues : unicellular and metazoan organisms used for molecular cell biology investigations -- Chemical foundations. Covalent bonds and noncovalent interactions -- Chemical building blocks of cells -- Chemical reactions and chemical equilibrium -- Biochemical energetics -- Protein structure and function. Hierarchical structure of proteins -- Protein folding -- Protein binding and enzyme catalysis -- Regulating protein function -- Purifying, detecting, and characterizing proteins -- Proteomics -- Part II. Genetics and molecular biology. Basic molecular genetic mechanism. Structure of nucleic acids -- Transcription of protein-coding genes and formation of functional mRNA -- The decoding of mRNA by tRNAs -- Stepwise synthesis of proteins on ribosomes -- DNA replication -- DNA repair and recombination -- Viruses : parasites of the cellular genetic system -- Molecular genetic techniques. Genetic analysis of mutations to identify and study genes -- DNA cloning and characterization -- Using cloned DNA fragments to study gene expression -- Locating and identifying human disease genes -- Inactivating the function of specific genes in eukaryotes -- Genes, genomics, and chromosomes. Eukaryotic gene structure -- Chromosomal organization of genes and noncoding DNA -- Transposable (mobile) DNA elements -- Organelle DNAs -- Genomics : genome-wide analysis of gene structure and expression -- Structural organization of eukaryotic chromosomes -- Morphology and functional elements of eukaryotic chromosomes -- Transcriptional control of gene expression. Control of gene expression in bacteria -- Overview of eukaryotic gene control -- RNA polymerase II promoters and general transcription factors -- Regulatory sequences in protein-coding genes and the proteins through which they function -- Molecular mechanisms of transcription repression and activation -- Regulation of transcription-factor activity -- Epigenetic regulation of transcription -- Other eukaryotic transcription systems -- Post-transcriptional gene control. Processing of eukaryotic pre-mRNA -- Regulation of pre-mRNA processing -- Transport of mRNA across the nuclear envelope -- Cytoplasmic mechanism of post-transcriptional control -- Processing of rRNA and tRNA -- Part III. Cell structure and function. Culturing, visualizing, and perturbing cells. Growing cells in culture -- Light microscopy : exploring cell structure and visualizing proteins within cells -- Electron microscopy : high-resolution imaging -- Isolation and characterization of cell organelles -- Perturbing specific cell functions -- Biomembrane structure. The lipid bilayer : composition and structural organization -- Membrane proteins : structure and basic functions -- Phospholipids, sphingolipids, and cholesterol : synthesis and intracellular movement -- Transmembrane transport of ions and small molecules. Overview of transmembrane transport -- Facilitated transport of glucose and water -- ATP-powered pumps and the intracellular ionic environment -- Nongated ion channels and the resting membrane potential -- Cotransport by symporters and antiporters -- Transcellular transport -- Cellular energetics. First step of harvesting energy from glucose : glycolysis -- Mitochondria and the citric acid cycle -- The electron transport chain and generation of the proton-motive force -- Harnessing the proton-motive force to synthesize ATP -- Photosynthesis and light-absorbing pigments -- Molecular analysis of photosystems -- CO2 metabolism during photosynthesis -- Moving proteins into membranes and organelles. Targeting proteins to and across the ER membrane -- Insertion of membrane proteins into the ER -- Protein modifications, folding, and quality control in the ER -- Targeting of proteins to mitochondria and chloroplasts -- Targeting of peroxisomal proteins -- Transport into and out of the nucleus -- Vesicular traffic, secretion, and endocytosis. Techniques for studying the secretory pathway -- Molecular mechanisms of vesicle budding and fusion -- Early stages of the secretory pathway -- Later stages of the secretory pathway -- Receptor-mediated endocytosis -- Directing membrane proteins and cytosolic materials to the lysosome -- Signal transduction and G protein-coupled receptors. Signal transduction : from extracellular signal to cellular response -- Studying cell-surface receptors and signal transduction proteins -- G protein-coupled receptors : structure and mechanism -- G protein-coupled receptors that regulate ion channels -- G protein-coupled receptors that activate or inhibit adenylyl cyclase -- G protein-coupled receptors that trigger elevations in cytosolic Ca2+ -- Signaling pathways that control gene expression. Receptors that activate protein tyrosine kinases -- The Ras/MAP kinase pathway -- Phosphoinositide signaling pathways -- Receptor serine kinases that activate smads -- Signaling pathways controlled by Ubiquitination : Wnt, Hedgehog, and NF-kb -- Signaling pathways controlled by protein cleavage : notch/delta, SREBP -- Integration of cellular responses to multiple signaling pathways -- Cell organization and movement I : microfilaments. Microfilaments and actin structures -- Dynamics of actin filaments -- Mechanisms of actin filament assembly -- Organization of actin-based cellular structures -- Myosins : actin-based motor proteins -- Myosin-powered movements -- Cell migration : mechanism, signaling, and chemotaxis -- Cell organization and movement II : microtubules and intermediate filaments. Microtubule structure and organization -- Microtubule dynamics -- Regulation of microtubule structure and dynamics -- Kinesins and dyneins : microtubule-based motor proteins -- Cilia and flagella : microtubule-based surface structures -- Mitosis -- Intermediate filaments -- Coordination and cooperation between cytoskeletal elements -- The eukaryotic cell cycle. Overview of the cell cycle and its control -- Model organisms and methods to study the cell cycle -- Regulation of CDK activity -- Commitment to the cell cycle and DNA replication -- Entry into mitosis -- Completion of mitosis : chromosome segregation and exit from mitosis -- Surveillance mechanisms in cell cycle regulation -- Meiosis : a special type of cell division -- Part IV. Cell growth and development. Integrating cells into tissues. Cell-cell and cell-matrix adhesion : an overview -- Cell-cell and cell-ECM junctions and their adhesion molecules -- The extracellular matrix I : the basal lamina -- The extracellular matrix II : connective tissue -- Adhesive interactions in motile and nonmotile cells -- Plant tissues -- Stem cells, cell asymmetry, and cell death. Early metazoan development and embryonic stem cells -- Stem cells and niches in multicellular organisms -- Mechanisms of cell polarity and asymmetric cell division -- Cell death and its regulation -- Nerve cells. Neurons and glia : building blocks of the nervous system -- Voltage-gated ion channels and the propagation of action potentials -- Communication at synapses -- Sensing the environment : touch, pain, taste, and smell -- Immunology. Overview of host defenses -- Immunoglobulins : structure and function -- Generation of antibody diversity and B-cell development -- The MHC and antigen presentation -- T cells, T-cell receptors, and T-cell development -- Collaboration of immune-system cells in the adaptive response -- Cancer. Tumor cells and the onset of cancer -- The genetic basis of cancer -- Cancer and misregulation of growth regulatory pathways -- Cancer and mutation of cell division and checkpoint regulators -- Carinogens and caretaker genes in cancer.

Overview: Molecular Cell Biology presents the key concepts in cell biology and their experimental underpinnings. The authors, all world-class researchers and teachers, incorporate medically relevant examples where appropriate to help illustrate the connections between cell biology and health and human disease. As always, a hallmark of MCB is the use of experiments to engage students in the history of cell biology and the research that has contributed to the field. The book contains predominantly color illustrations, with some black-and-white illustrations.